2) The precipitated protein can be difficult to resuspend and often cannot be fully recovered.
Protocol Introduction The 2-D, clean, backup disk image windows 7 up, kit can be used on virtually any protein sample.
Co-precipitant: This solution contains reagents that co-precipitate with proteins and enhances their removal from solution.Overview The ability to analyze a sample effectively by 2-D electrophoresis often limited by the presence of non-protein impurities in the sample.If desired, sample proteins may be extracted from cells or solid tissues using the Sample Grinding.Do not use internally or externally in humans or animals.I am attaching the Protocol, and the product information.The procedure works by quantitatively precipitating proteins while leaving behind in solution interfering substances such as detergents, salts, lipids, phenolics and nucleic acids.Kit and a solubilizing solution consisting of 2 sodium dodecylsulfate (SDS 40 mM Tris base and 60 mM dithiothreitol (DTT).The kit can enable effective 2-D analysis of samples that are otherwise too dirty or dilute.Treatment of the sample with the 2-D, clean, up, kit can improve the quality of 2-D electrophoresis results, reducing streaking, background staining and other consequences of interfering contaminants.In the case of contact with skin or eyes wash immediately with water.The procedure does not result in spot gain or loss, or changes in spot position relative to untreated samples.It is a long link, just copy paste all.The mixture is centrifuged again and the resultant pellet can be easily resuspended into a 2-D sample solution of choice.GE, healthcare 2-D, clean, up, kit, product Specification Sheet The 2-D, clean, up, kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D results due to high conductivity, high levels of interfering substances or low md 8818 bios update concentration.Not recommended or intended for diagnosis of disease in humans or animals.
The procedure can be completed in one hour and does not result in spot gain or loss.
The kit contains sufficient reagents to process 50 samples of up to 100 l each.